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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene continues to be observed in several tumors, which leads to chromosome instability, and therefore could lead to tumorigenesis. This protein was located to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a vital role in promoting mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two more splice variants had been reported but comprehensive nucleotide sequence information is not but accessible.
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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide generally includes the epitope regarded from the antibody.
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This manual outlines even more website achievable optimization of mobile and immunoassay parameters to make sure the absolute best results are...
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This guideline outlines even more achievable optimization of mobile and immunoassay parameters to make certain the very best results are...
Antibodies sure to the blocking peptide not bind to your epitope over the goal protein. By comparing the staining from the blocked antibody compared to the antibody on your own, you can see which staining is precise.
Concentrate on Details Cyclin E belongs to the very conserved cyclin spouse and children, whose members are characterised by a extraordinary periodicity in protein abundance through the cell cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit unique expression and degradation patterns which contribute towards the temporal coordination of each mitotic function. Cyclin E types a fancy with and functions as being a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S transition. This protein accumulates for the G1-S period boundary and is particularly degraded as cells development by S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both of those antibodies...
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